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Johns Hopkins Center for TB Research Johns Hopkins School of Medicine

TARGET now has more than 4300 unique transposon mutants!!!
Click here to browse our mutant collection
Click here to order mutants from BEI
Search our Mutants

Latest Hits!!!
Rv1874 Rv0241c
Rv2565 Rv2417c
Rv0584 Rv2258c
Updated May 12, 2009
Details
DescriptionPROBABLE FATTY-ACID-CoA LIGASE FADD34 (FATTY-ACID-CoA SYNTHETASE) (FATTY-ACID-CoA SYNTHASE)
Accession numberRv0035
Gene namefadD34
Gene length1689
Protein length562
Coordinates37259..38947
Orientation+
Molecular mass60961.38
Isoelectric point6.47

Database Crossreferences
Deletion Mutant Phenotype
in-vivoguinea pig
 mouse
 rabbit

in-vitroHBMEC
 macrophage

Mutant Survival in DeADMAn (Lamichhane et al., 2005)*

Strain NamePhenotype (Mouse)
JHU0035-103Not-Attenuated
JHU0035-494[no data]
JHU0035-1331[no data]
JHU0035-525[no data]
JHU0035-526[no data]
 

CDC1551 Homolog
ORFMT0040
Essentiality Prediction
Sassetti et al, 2003*non-essential

JHU Transposon Mutant(s) Available
NameMT#Rv#POIGene LengthAvailable from BEI
JHU0035-494MT0040Rv00354941689no (will be available later)
JHU0035-526MT0040Rv00355261689yes
  
For a list of currently available mutants, please visit BEI's transposon mutant webpage.

If we have not disrupted your gene using random mutagenesis, we may be able to create a knockout mutant for you.  Please click here  to submit a request.

References
Lamichhane, G., M. Zignol, N. J. Blades, D. E. Geiman, A. Dougherty, J. Grosset, K. W. Broman, and W. R. Bishai. (2003) Proc. Natl. Acad. Sci. USA 100:7213-7218. [Abstract/Free Full Text]
Sassetti, C. M., Boyd, D. H. & Rubin, E. J. (2003) Proc. Natl. Acad. Sci. USA 100:12989-12994. [Abstract/Free Full Text]
Lamichhane G, Tyagi S, Bishai WR (2005) Infect Immun. Apr;73(4):2533-40.
[Abstract/Free Full Text]