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Johns Hopkins Center for TB Research Johns Hopkins School of Medicine

TARGET now has more than 4300 unique transposon mutants!!!
Click here to browse our mutant collection
Click here to order mutants from BEI
Search our Mutants

Latest Hits!!!
Rv1874 Rv0241c
Rv2565 Rv2417c
Rv0584 Rv2258c
Updated May 12, 2009
Details
DescriptionMEMBRANE-ANCHORED ADENYLYL CYCLASE CYA (ATP PYROPHOSPHATE-LYASE) (ADENYLATE CYCLASE)
Accession numberRv1625c
Gene namecya
Gene length1332
Protein length443
Coordinates1826614..1827945
Orientation-
Molecular mass47337.38
Isoelectric point6.18

Database Crossreferences
Deletion Mutant Phenotype
in-vivoguinea pig
 mouse
 rabbit

in-vitroHBMEC
 macrophage

Mutant Survival in DeADMAn (Lamichhane et al., 2005)*

Strain NamePhenotype (Mouse)
JHU1625c-795[no data]
JHU1625c-1244[no data]
JHU1625c-1259[no data]
JHU1625c-278[no data]
JHU1625c-1091[no data]
JHU1625c-796[no data]
JHU1625c-1222[no data]
 

CDC1551 Homolog
ORFMT1661
Essentiality Prediction
Sassetti et al, 2003*non-essential

JHU Transposon Mutant(s) Available
NameMT#Rv#POIGene LengthAvailable from BEI
JHU1625c-795MT1661Rv1625c7951332yes
JHU1625c-1244MT1661Rv1625c12441332no (will be available later)
JHU1625c-1091MT1661Rv1625c10911332no (will be available later)
JHU1625c-796MT1661Rv1625c7961332yes
JHU1625c-1222MT1661Rv1625c12221332yes
  
For a list of currently available mutants, please visit BEI's transposon mutant webpage.

If we have not disrupted your gene using random mutagenesis, we may be able to create a knockout mutant for you.  Please click here  to submit a request.

References
Lamichhane, G., M. Zignol, N. J. Blades, D. E. Geiman, A. Dougherty, J. Grosset, K. W. Broman, and W. R. Bishai. (2003) Proc. Natl. Acad. Sci. USA 100:7213-7218. [Abstract/Free Full Text]
Sassetti, C. M., Boyd, D. H. & Rubin, E. J. (2003) Proc. Natl. Acad. Sci. USA 100:12989-12994. [Abstract/Free Full Text]
Lamichhane G, Tyagi S, Bishai WR (2005) Infect Immun. Apr;73(4):2533-40.
[Abstract/Free Full Text]