target banner
Johns Hopkins Center for TB Research Johns Hopkins School of Medicine

TARGET now has more than 4300 unique transposon mutants!!!
Click here to browse our mutant collection
Click here to order mutants from BEI
Search our Mutants

Latest Hits!!!
Rv1874 Rv0241c
Rv2565 Rv2417c
Rv0584 Rv2258c
Updated May 12, 2009
Details
DescriptionPROBABLE MEMBRANE-ASSOCIATED PHOSPHOLIPASE C 1 PLCA (MTP40 ANTIGEN)
Accession numberRv2351c
Gene nameplcA
Gene length1539
Protein length512
Coordinates2630537..2632075
Orientation-
Molecular mass55381.03
Isoelectric point6.43

Database Crossreferences
Deletion Mutant Phenotype
in-vivoguinea pig
 mouse
 rabbit

in-vitroHBMEC
 macrophage

Mutant Survival in DeADMAn (Lamichhane et al., 2005)*

Strain NamePhenotype (Mouse)
JHU2351c-588[no data]
JHU2351c-548[no data]
JHU2351c-588Not-Attenuated
JHU2351c-461[no data]
JHU2351c-778[no data]
JHU2351c-485[no data]
JHU2351c-1041[no data]
 

CDC1551 Homolog
ORFMT2416
Essentiality Prediction
Sassetti et al, 2003*[no data]

JHU Transposon Mutant(s) Available
NameMT#Rv#POIGene LengthAvailable from BEI
JHU2351c-588MT2416Rv2351c5881563no (will be available later)
JHU2351c-548MT2416Rv2351c5481563yes
JHU2351c-461MT2416Rv2351c4611563yes
JHU2351c-778MT2416Rv2351c7781563no (will be available later)
JHU2351c-485MT2416Rv2351c4851563yes
  
For a list of currently available mutants, please visit BEI's transposon mutant webpage.

If we have not disrupted your gene using random mutagenesis, we may be able to create a knockout mutant for you.  Please click here  to submit a request.

References
Lamichhane, G., M. Zignol, N. J. Blades, D. E. Geiman, A. Dougherty, J. Grosset, K. W. Broman, and W. R. Bishai. (2003) Proc. Natl. Acad. Sci. USA 100:7213-7218. [Abstract/Free Full Text]
Sassetti, C. M., Boyd, D. H. & Rubin, E. J. (2003) Proc. Natl. Acad. Sci. USA 100:12989-12994. [Abstract/Free Full Text]
Lamichhane G, Tyagi S, Bishai WR (2005) Infect Immun. Apr;73(4):2533-40.
[Abstract/Free Full Text]